Links

HPLC Simulation

This simulator running in Java is quite sophisticated, yet easy to use.

Instument Configuration Simulation

This Fluid Visualizer also running in Java, allows drag-and-drop building of virtual HPLC instruments, and provides estimates of pressure drops and dispersion values.

Choosing HPLC columns with Orthogonal/Similar Selectivity

See www.hplccolumns.org ‐ this site is a complement to the USP website on the same topic, and provides estimates of column similarity using stationary phase characterization data based on the Hydophobic Subtraction Model of reversed-phase selectivity.

Orthogonality Estimation from Experimental Data

OrCa ‐ Windows-based software that calculates orthogonality of a 2D separation using fractals. This is based on the work of Mark Schure, which is described in a paper in the Journal of Chromatography, A.

Multi-Dimensional Separations

A site dedicated to multi-dimensional separations with an emphasis on the liquid phase.

HPLC Toolkit

Below are the links to several tools that can be used to aid in HPLC calculations. The bar on the left contains links to additional HPLC simulators and tools

Basics	Tubing Volume	Viscosity Calculator
Dynamics-Level 1	Viscosity	Diffusion Coefficients	Partical Size Crossover	Gradients	Method Transfer
Dynamics-Level 2	Dynamics Dashboard	Poppe Plot-Isocratic	Poppe Plot-Gradient	Compare Poppe Curves	Pressure Prediction Plot
2D Liquid Chromatography	Fraction Volume
LC Mass Spec