HPLC Simulator
| Name | ||
|---|---|---|
| ACN | MeOH | |
| lnkw int. | ||
| lnkw slope | ||
| S int. | ||
| S slope | ||
| Compounds | Concentration | |
|---|---|---|
| Solvent A | |||
|---|---|---|---|
| Solvent B | |||
| Isocratic Elution Mode | |||
| Gradient Elution Mode | |||
| Solvent B Fraction (% v/v) | |||
| Time (min) | % B |
|---|---|
Pre-column volume:
| Gradient Delay Volume: | μL | |
| Dwell time: | 0.10 | min |
| Temperature | ||
|---|---|---|
| °C | ||
| Injection Volume | μL | |
| Flow Rate | mL/min | |
| Flow Velocities | ||
| Open Tube | 0.2006 | cm/s |
| Interstitial | 0.5014 | cm/s |
| Chromatographic | 0.3134 | cm/s |
| Reduced | 12.8697 | |
| H (plate height) | 6.0960e-4 | cm |
| N (plate number) | 16404 | |
| Backpressure | 147.98 | bar |
| Eluent Viscosity | 0.6558 | cP |
|---|---|---|
| Avg. Diffusion Coeff. | 1.1689e-5 | cm2/s |
| Signal Offset | munits | |
| Noise | ||
| Time Constant | s | |
| Automatically determine time span | ||
| Initial Time | s | |
| Final Time | s | |
| Plot Points | ||
| Detector Frequency | Hz | |
| Data Point Count | 382 | |
| Plot points on graph | ||
| Length | cm | |
| Inner Diameter | mil | |
| Volume | 0.0000 | μL |
| Stationary Phase | |||
|---|---|---|---|
| Length | mm | ||
| Inner Diameter | mm | ||
| Particle Size | μm | ||
| Interparticle porosity | |||
| Intraparticle porosity | |||
| Total Porosity | 0.6400 | ||
| Void Volume | 1.0636 | mL | |
| Void Time | 31.9085 | s | |
| Van Deemter Parameters | |||
| A | |||
| B | |||
| C | |||
| Reduced plate height | 2.0320 | ||
| Compound | k | tR (min) | σ (s) | kw | S |
|---|
| phenol | 1.4343 | 1.2946 | 0.6162 | 8.7216 | 4.5128 |
| benzonitrile | 3.1903 | 2.2285 | 1.0496 | 27.2133 | 5.3590 |
| p-chlorophenol | 3.2584 | 2.2646 | 1.0665 | 40.7610 | 6.3163 |
| acetophenone | 3.6169 | 2.4553 | 1.1554 | 45.8185 | 6.3477 |
| nitrobenzene | 4.6317 | 2.9950 | 1.4073 | 49.7395 | 5.9347 |